User:Bohao Liu/Notebook/Research/2014/04/29: Difference between revisions
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== | ==HOG Experiments : wild - type controls== | ||
===Objective=== | |||
*Make sure I can do HOG experiments again | |||
*Confirm Wild-type values and also expand the frequencies tested | |||
*Confirm the protocol: specifically that waiting 30 minutes between each set of oscillations is enough to ensure that the cells return to normal | |||
*See if the cells are growing | |||
===Experiment=== | |||
*Cells OD @ 10:30 = .389 | |||
*Started flow at 11 am | |||
*Started experiment at 11:30 am | |||
====Settings==== | |||
*240 second half-period | |||
*48 second imaging = 6 images per cycle | |||
*40 minute total time = 5 complete periods | |||
*ND=2 | |||
*mCherry = 100 mS exposure | |||
*GFP = 200 mS exposure | |||
*DIC | |||
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Revision as of 08:31, 29 April 2014
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HOG Experiments : wild - type controlsObjective
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