User:Bohao Liu/Notebook/Research/2014/04/29

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(Autocreate 2014/04/29 Entry for User:Bohao_Liu/Notebook/Research)
Current revision (11:55, 6 May 2014) (view source)
(HOG Experiments : wild - type controls)
 
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==Title==
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==HOG Experiments : wild - type controls==
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===Smaller Title===
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===Objective===
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*Stuff
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*Make sure I can do HOG experiments again
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#Numbered stuff
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*Confirm Wild-type values and also expand the frequencies tested
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*Confirm the protocol: specifically that waiting 30 minutes between each set of oscillations is enough to ensure that the cells return to normal
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*See if the cells are growing
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===Experiment===
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*Cells OD  @ 10:30 = .389
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*Started flow at 11 am
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*Started experiment at 11:30 am
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====Settings====
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*240 second half-period
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*48 second imaging = 6 images per cycle
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*40 minute total time = 5 complete periods
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*ND=2
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*mCherry = 200 mS exposure
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*GFP = 200 mS exposure
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*DIC
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====Cells====
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*1:200 from 1.5 OD is at .973 OD 24 hours later
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*1:100 set back 16 hours is at ~ .400 OD
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HOG Experiments : wild - type controls

Objective

  • Make sure I can do HOG experiments again
  • Confirm Wild-type values and also expand the frequencies tested
  • Confirm the protocol: specifically that waiting 30 minutes between each set of oscillations is enough to ensure that the cells return to normal
  • See if the cells are growing

Experiment

  • Cells OD @ 10:30 = .389
  • Started flow at 11 am
  • Started experiment at 11:30 am

Settings

  • 240 second half-period
  • 48 second imaging = 6 images per cycle
  • 40 minute total time = 5 complete periods
  • ND=2
  • mCherry = 200 mS exposure
  • GFP = 200 mS exposure
  • DIC


Cells

  • 1:200 from 1.5 OD is at .973 OD 24 hours later
  • 1:100 set back 16 hours is at ~ .400 OD



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