User:Bohao Liu/Notebook/Research/2014/04/29: Difference between revisions

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==Title==
==HOG Experiments : wild - type controls==




===Smaller Title===
===Objective===
*Stuff
*Make sure I can do HOG experiments again
#Numbered stuff
*Confirm Wild-type values and also expand the frequencies tested
*Confirm the protocol: specifically that waiting 30 minutes between each set of oscillations is enough to ensure that the cells return to normal
*See if the cells are growing
 
===Experiment===
*Cells OD  @ 10:30 = .389
*Started flow at 11 am
*Started experiment at 11:30 am
 
====Settings====
*240 second half-period
*48 second imaging = 6 images per cycle
*40 minute total time = 5 complete periods
 
*ND=2
*mCherry = 200 mS exposure
*GFP = 200 mS exposure
*DIC
 
 
 
====Cells====
*1:200 from 1.5 OD is at .973 OD 24 hours later
*1:100 set back 16 hours is at ~ .400 OD
 


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Latest revision as of 23:56, 26 September 2017

Project name Main project page
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HOG Experiments : wild - type controls

Objective

  • Make sure I can do HOG experiments again
  • Confirm Wild-type values and also expand the frequencies tested
  • Confirm the protocol: specifically that waiting 30 minutes between each set of oscillations is enough to ensure that the cells return to normal
  • See if the cells are growing

Experiment

  • Cells OD @ 10:30 = .389
  • Started flow at 11 am
  • Started experiment at 11:30 am

Settings

  • 240 second half-period
  • 48 second imaging = 6 images per cycle
  • 40 minute total time = 5 complete periods
  • ND=2
  • mCherry = 200 mS exposure
  • GFP = 200 mS exposure
  • DIC


Cells

  • 1:200 from 1.5 OD is at .973 OD 24 hours later
  • 1:100 set back 16 hours is at ~ .400 OD



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