User:Bohao Liu/Notebook/Research/2014/04/30

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(Autocreate 2014/04/30 Entry for User:Bohao_Liu/Notebook/Research)
Current revision (14:30, 30 April 2014) (view source)
(HOG Experiments : wild - type controls and Image Analysis Parameters)
 
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==Title==
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==HOG Experiments : wild - type controls and Image Analysis Parameters==
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===Objective===
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*Make sure I can do HOG experiments again
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*Confirm Wild-type values and also expand the frequencies tested
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*Confirm the protocol: specifically that waiting 30 minutes between each set of oscillations is enough to ensure that the cells return to normal
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*See if the cells are growing
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===Experiment===
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*Cells OD  @ 10 = .453
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*Started flow at 10:45 am
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*Started experiment at 11:15 am
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====Settings====
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*30 second half-period
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*6 second imaging = 6 images per cycle
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*5 minute total time = 5 complete periods
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*30 minutes in between for a total of 5 sets
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*1 hour rest after this set of 5
 +
 
 +
*30 second half-period
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*6 second imaging = 6 images per cycle
 +
*20 minute total time = 20 complete periods
 +
 
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*ND=2
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*mCherry = 200 mS exposure
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*GFP = 200 mS exposure
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*DIC
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====Image Analysisp====
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*for windows sorting :
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**<code>for /l %x in (1,1,5) do MD well%x</code>
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**<code>for /l %x in (1,1,5) do MOVE col*t*xy%xc*.tif well%x</code>
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===Smaller Title===
 
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*Stuff
 
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#Numbered stuff
 
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HOG Experiments : wild - type controls and Image Analysis Parameters

Objective

  • Make sure I can do HOG experiments again
  • Confirm Wild-type values and also expand the frequencies tested
  • Confirm the protocol: specifically that waiting 30 minutes between each set of oscillations is enough to ensure that the cells return to normal
  • See if the cells are growing

Experiment

  • Cells OD @ 10 = .453
  • Started flow at 10:45 am
  • Started experiment at 11:15 am

Settings

  • 30 second half-period
  • 6 second imaging = 6 images per cycle
  • 5 minute total time = 5 complete periods
  • 30 minutes in between for a total of 5 sets
  • 1 hour rest after this set of 5
  • 30 second half-period
  • 6 second imaging = 6 images per cycle
  • 20 minute total time = 20 complete periods
  • ND=2
  • mCherry = 200 mS exposure
  • GFP = 200 mS exposure
  • DIC


Image Analysisp

  • for windows sorting :
    • for /l %x in (1,1,5) do MD well%x
    • for /l %x in (1,1,5) do MOVE col*t*xy%xc*.tif well%x




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