User:Bohao Liu/Notebook/Research/2014/04/30

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(HOG Experiments : wild - type controls)
Current revision (14:30, 30 April 2014) (view source)
(HOG Experiments : wild - type controls and Image Analysis Parameters)
 
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====Settings====
====Settings====
*30 second half-period
*30 second half-period
-
*5 second imaging = 6 images per cycle
+
*6 second imaging = 6 images per cycle
*5 minute total time = 5 complete periods
*5 minute total time = 5 complete periods
 +
*30 minutes in between for a total of 5 sets
 +
 +
*1 hour rest after this set of 5
 +
 +
*30 second half-period
 +
*6 second imaging = 6 images per cycle
 +
*20 minute total time = 20 complete periods
*ND=2
*ND=2
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*GFP = 200 mS exposure
*GFP = 200 mS exposure
*DIC
*DIC
 +
 +
====Image Analysisp====
====Image Analysisp====
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**<code>for /l %x in (1,1,5) do MD well%x</code>
**<code>for /l %x in (1,1,5) do MD well%x</code>
**<code>for /l %x in (1,1,5) do MOVE col*t*xy%xc*.tif well%x</code>
**<code>for /l %x in (1,1,5) do MOVE col*t*xy%xc*.tif well%x</code>
-
 

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HOG Experiments : wild - type controls and Image Analysis Parameters

Objective

  • Make sure I can do HOG experiments again
  • Confirm Wild-type values and also expand the frequencies tested
  • Confirm the protocol: specifically that waiting 30 minutes between each set of oscillations is enough to ensure that the cells return to normal
  • See if the cells are growing

Experiment

  • Cells OD @ 10 = .453
  • Started flow at 10:45 am
  • Started experiment at 11:15 am

Settings

  • 30 second half-period
  • 6 second imaging = 6 images per cycle
  • 5 minute total time = 5 complete periods
  • 30 minutes in between for a total of 5 sets
  • 1 hour rest after this set of 5
  • 30 second half-period
  • 6 second imaging = 6 images per cycle
  • 20 minute total time = 20 complete periods
  • ND=2
  • mCherry = 200 mS exposure
  • GFP = 200 mS exposure
  • DIC


Image Analysisp

  • for windows sorting :
    • for /l %x in (1,1,5) do MD well%x
    • for /l %x in (1,1,5) do MOVE col*t*xy%xc*.tif well%x




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