The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>
|
HOG Experiments : wild - type controls and Image Analysis Parameters
Objective
- Make sure I can do HOG experiments again
- Confirm Wild-type values and also expand the frequencies tested
- Confirm the protocol: specifically that waiting 30 minutes between each set of oscillations is enough to ensure that the cells return to normal
- See if the cells are growing
Experiment
- Cells OD @ 10 = .453
- Started flow at 10:45 am
- Started experiment at 11:15 am
Settings
- 30 second half-period
- 5 second imaging = 6 images per cycle
- 5 minute total time = 5 complete periods
- ND=2
- mCherry = 200 mS exposure
- GFP = 200 mS exposure
- DIC
Image Analysisp
- for windows sorting :
for /l %x in (1,1,5) do MD well%xfor /l %x in (1,1,5) do MOVE col*t*xy%xc*.tif well%x
|
Project name
