User:Brendan F. Fries/Notebook/G-Bioscience Extraction of Genomic DNA from LUC-14 Cells

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(G-Biosciences XIT Genomic DNA extraction from cultured cells)
Current revision (05:13, 27 February 2014) (view source)
(G-Biosciences XIT Genomic DNA extraction from cultured cells)
 
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[http://openwetware.org/wiki/User:Brendan_F._Fries/Notebook/G-Bioscience_Extraction_of_Genomic_DNA_from_LUC-14_Cells/2014/02/22 2/22/14]
[http://openwetware.org/wiki/User:Brendan_F._Fries/Notebook/G-Bioscience_Extraction_of_Genomic_DNA_from_LUC-14_Cells/2014/02/22 2/22/14]
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==Notes==
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*Centrifuge times were low and more centrifuging was required to fully separate the supernatant from the precipitate.
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*White object present in medium, DNA levels were still good regardless.
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G-Biosciences XIT Genomic DNA extraction from cultured cells

  • LUC-14 cells cultured in 6-well plate

2/19/14

  • Genomic DNA extraction from cells

2/21/14

  • DNA concentration measured

2/22/14

Notes

  • Centrifuge times were low and more centrifuging was required to fully separate the supernatant from the precipitate.
  • White object present in medium, DNA levels were still good regardless.

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