User:Brian P. Josey/Notebook/2009/09/10: Difference between revisions
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== | ==GTP Magnesium Salt and Questions== | ||
Andy had me look for GTP magnesium salt, but I could not find any. We decide that since we need the magnesium in solution, we could use buffers with a little bit of magnesium chloride, MgCl<sub>2</sub>, in solution. | |||
We were also talking about why we using GTP in the first place. Essentially, when the tubulin dimers polymerize into the microtubules GDP rest in the center of one of the dimers. Another one comes to the side, and some how it uses magnesium ions to create a chain of tubulin dimers creating the microtubule. | |||
I asked a couple of questions, and I'm leaving them here so I have a reference somewhere that includes my questions about what we are doing. | |||
*How does the tubulin use magnesium? | |||
*Does the concentration of magnesium decrease? | |||
*How fast do the tubulin polymerize into microtubules? | |||
*Why does the kinesin go toward the positive end? | |||
I have an idea on some of these, but I'm going to do some more research into them to see what I can find out, and maybe find a topic or two to investigate on my own. | |||
Revision as of 14:08, 10 September 2009
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GTP Magnesium Salt and QuestionsAndy had me look for GTP magnesium salt, but I could not find any. We decide that since we need the magnesium in solution, we could use buffers with a little bit of magnesium chloride, MgCl2, in solution. We were also talking about why we using GTP in the first place. Essentially, when the tubulin dimers polymerize into the microtubules GDP rest in the center of one of the dimers. Another one comes to the side, and some how it uses magnesium ions to create a chain of tubulin dimers creating the microtubule. I asked a couple of questions, and I'm leaving them here so I have a reference somewhere that includes my questions about what we are doing.
I have an idea on some of these, but I'm going to do some more research into them to see what I can find out, and maybe find a topic or two to investigate on my own.
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