- Bryan Mejia-Sosa
- Massachusetts Institute of Technology
- 351 Massachusetts Ave
- Cambridge, Massachusetts, United States of America
- Email me through OpenWetWare
I work in the Lauffenburger Lab at MIT. I learned about OpenWetWare from my current MIT Biological Engineering Lab Course, and I've joined because I want to learn more about bioengineering lab protocols, but more so about the biological/chemical mechanisms behind such processes.
- MIT Class 2014
- MicroRNA applications
Registration/Questionnaire: 20.109 Fall 2012
Year of Graduation
bmejiaso AT mit DOT edu
Have you taken or are you taking...
20.320 (Cell Kinetics): currently taking
7.05/5.07 (Biochemistry): completed
7.06 (Cell Biology): have not taken
5.310 (General Chemistry Lab): have not taken
Do you have any experience culturing cells (mammalian, yeast or microbial)?
Yes, with mammalian and microbial cells
Do you have any experience in molecular biology (electrophoresis, PCR, etc)?
Please briefly describe any previous laboratory experience
- UROP in the Lauffenburger Lab: I am currently working with HCC cell lines and how microRNA expression levels in these cells have a role in regulating cell survival decisions combined with cytokine treatment. This includes, but not limited to qPCR amplification and quantification, cell culture and passaging, and plasmid design.
- UROP in Zhang Lab: I worked on elucidating the protein structure of specific Drosophila olfactory receptors through cell-free in vitro translation protein production. This included, but not limited to plasmid recombination, PCR amplification, and cell transformation.
Please briefly describe any previous technical communication experience you have
- Written Communication: I have written analyses on projects, both for UROPs and for other technical projects that I've worked on in the past.
- Oral Communication: I have presented powerpoint presentations on my findings in previous projects to my peers. I have also discussed the intricacies of my projects with my supervisor on a daily basis.
Anything else you would like us to know?
Always excited to learn more!