User:Burak Yilmaz/Notebook/YR10 protein interaction/2009/08/31: Difference between revisions
From OpenWetWare
Burak Yilmaz (talk | contribs) |
Burak Yilmaz (talk | contribs) |
||
| Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==Primer design for arabidopsis GCN2 gene == | ===Primer design for arabidopsis GCN2 gene === | ||
==forward primer== | |||
5' GGATTAATTAAATGGACTACAAGGACGACGATGACAAAGTCAAGCTTCTCGAGAATTCCGGTCGCAGCAGTTCGAAGAAAAAGAAGAA 3' | * 5' GGATTAATTAAATGGACTACAAGGACGACGATGACAAAGTCAAGCTTCTCGAGAATTCCGGTCGCAGCAGTTCGAAGAAAAAGAAGAA 3' | ||
LENGTH: 77 | * LENGTH: 77 | ||
GC CONTENT: 45.5 % | * GC CONTENT: 45.5 % | ||
MELT TEMP: 69.9 ºC | * MELT TEMP: 69.9 ºC | ||
MOLECULAR | * MOLECULAR | ||
WEIGHT: 23870.5 g/mole | * WEIGHT: 23870.5 g/mole | ||
reverse primer | ==reverse primer== | ||
5' GGTGCGGCCGCTTAGCTCCAAACAGAGGGGTTTCTAAATTG 3' | *5' GGTGCGGCCGCTTAGCTCCAAACAGAGGGGTTTCTAAATTG 3' | ||
LENGTH: 41 | *LENGTH: 41 | ||
GC CONTENT: 53.7 % | *GC CONTENT: 53.7 % | ||
MELT TEMP: 68.8 ºC | *MELT TEMP: 68.8 ºC | ||
MOLECULAR | *MOLECULAR | ||
WEIGHT: 12681.2 g/mole | *WEIGHT: 12681.2 g/mole | ||
TTAATTAA PacI site | *TTAATTAA PacI site | ||
GACTACAAGGACGACGATGACAAA Flag tag | *GACTACAAGGACGACGATGACAAA Flag tag | ||
GCGGCCGC NotI site | *GCGGCCGC NotI site | ||
Revision as of 12:31, 3 September 2009
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Primer design for arabidopsis GCN2 geneforward primer
reverse primer
| |
