User:Cameron Neylon

Cameron Neylon School of Chemistry University of Southampton Southampton United Kingdom SO17 1BJ C.Neylon@rl.ac.uk

I am a Lecturer in Chemical Biology at the School of Chemistry at the University of Southampton with a joint appointment at the ISIS Pulsed Neutron Source, Rutherford Appleton Laboratory, near Oxford. The group at Southampton works in the areas of protein-ligand binding and directed evolution with a focus on methods development for high throughput analysis and protein labeling. At the Rutherford Lab we are developing and promoting neutron and X-ray scattering techniques for applications in the biological sciences.

The combination of high throughput methodology and working on two sites has led to a developing programme in e-science particularly in the area of electronic lab notebooks.

Research Interests

• Methods for selective protein labelling
• Directed evolution
• High throughput methods for genotype and phenotype analysis
• Small angle X-ray and Neutron scattering
• Electronic lab notebooks and automatic data capture

Publications

1. Mulcair MD, Schaeffer PM, Oakley AJ, Cross HF, Neylon C, Hill TM, and Dixon NE. A molecular mousetrap determines polarity of termination of DNA replication in E. coli. Cell. 2006 Jun 30;125(7):1309-19. DOI:10.1016/j.cell.2006.04.040 |
2. Whiteford N, Haslam N, Weber G, Prügel-Bennett A, Essex JW, Roach PL, Bradley M, and Neylon C. An analysis of the feasibility of short read sequencing. Nucleic Acids Res. 2005 Nov 7;33(19):e171. DOI:10.1093/nar/gni170 |

3. Weber G, Haslam N, Whiteford N, Prugel-Bennett A, Essex JW, and Neylon C. Thermal equivalence of DNA duplexes without calculation of melting temperature. Nature Physics, 2(1), 55-59, doi:10.1038/nphys189

4. Neylon C, Kralicek AV, Hill TM, and Dixon NE. Replication termination in Escherichia coli: structure and antihelicase activity of the Tus-Ter complex. Microbiol Mol Biol Rev. 2005 Sep;69(3):501-26. DOI:10.1128/MMBR.69.3.501-526.2005 |
5. Wood RJ, Pascoe DD, Brown ZK, Medlicott EM, Kriek M, Neylon C, and Roach PL. Optimized conjugation of a fluorescent label to proteins via intein-mediated activation and ligation. Bioconjug Chem. 2004 Mar-Apr;15(2):366-72. DOI:10.1021/bc0341728 |
6. Neylon C. Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolution. Nucleic Acids Res. 2004;32(4):1448-59. DOI:10.1093/nar/gkh315 |
7. Neylon C, Brown SE, Kralicek AV, Miles CS, Love CA, and Dixon NE. Interaction of the Escherichia coli replication terminator protein (Tus) with DNA: a model derived from DNA-binding studies of mutant proteins by surface plasmon resonance. Biochemistry. 2000 Oct 3;39(39):11989-99. DOI:10.1021/bi001174w |