User:Cameron Neylon
Cameron Neylon School of Chemistry C.Neylon@rl.ac.uk
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I am a Lecturer in Chemical Biology at the School of Chemistry at the University of Southampton with a joint appointment at the ISIS Pulsed Neutron Source, Rutherford Appleton Laboratory, near Oxford. The group at Southampton works in the areas of protein-ligand binding and directed evolution with a focus on methods development for high throughput analysis and protein labeling. At the Rutherford Lab we are developing and promoting neutron and X-ray scattering techniques for applications in the biological sciences.
The combination of high throughput methodology and working on two sites has led to a developing programme in e-science particularly in the area of electronic lab notebooks.
Research Interests
- Methods for selective protein labelling
- Directed evolution
- High throughput methods for genotype and phenotype analysis
- Small angle X-ray and Neutron scattering
- Electronic lab notebooks and automatic data capture
Publications
- Cavalli G, Banu S, Ranasinghe RT, Broder GR, Martins HF, Neylon C, Morgan H, Bradley M, and Roach PL. Multistep synthesis on SU-8: combining microfabrication and solid-phase chemistry on a single material. J Comb Chem. 2007 May-Jun;9(3):462-72. DOI:10.1021/cc060079p |
- Mulcair MD, Schaeffer PM, Oakley AJ, Cross HF, Neylon C, Hill TM, and Dixon NE. A molecular mousetrap determines polarity of termination of DNA replication in E. coli. Cell. 2006 Jun 30;125(7):1309-19. DOI:10.1016/j.cell.2006.04.040 |
- Whiteford N, Haslam N, Weber G, Prügel-Bennett A, Essex JW, Roach PL, Bradley M, and Neylon C. An analysis of the feasibility of short read sequencing. Nucleic Acids Res. 2005 Nov 7;33(19):e171. DOI:10.1093/nar/gni170 |
Weber G, Haslam N, Whiteford N, Prugel-Bennett A, Essex JW, and Neylon C. Thermal equivalence of DNA duplexes without calculation of melting temperature. Nature Physics, 2(1), 55-59, doi:10.1038/nphys189
- Neylon C, Kralicek AV, Hill TM, and Dixon NE. Replication termination in Escherichia coli: structure and antihelicase activity of the Tus-Ter complex. Microbiol Mol Biol Rev. 2005 Sep;69(3):501-26. DOI:10.1128/MMBR.69.3.501-526.2005 |
- Wood RJ, Pascoe DD, Brown ZK, Medlicott EM, Kriek M, Neylon C, and Roach PL. Optimized conjugation of a fluorescent label to proteins via intein-mediated activation and ligation. Bioconjug Chem. 2004 Mar-Apr;15(2):366-72. DOI:10.1021/bc0341728 |
- Neylon C. Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolution. Nucleic Acids Res. 2004;32(4):1448-59. DOI:10.1093/nar/gkh315 |
- Neylon C, Brown SE, Kralicek AV, Miles CS, Love CA, and Dixon NE. Interaction of the Escherichia coli replication terminator protein (Tus) with DNA: a model derived from DNA-binding studies of mutant proteins by surface plasmon resonance. Biochemistry. 2000 Oct 3;39(39):11989-99. DOI:10.1021/bi001174w |