User:Carly M. Montanero/Notebook/CHEM-571/2013/08/28: Difference between revisions
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==Calculations from Part 2== | ==Calculations from Part 2== | ||
* The absorbance of the citrate-AuNP solution was determined by first determining the absorbance of a blank sample. The absorbance of the citrate-AuNP solution was correct by subtracting the absorbance of the blank sample. | * The absorbance of the citrate-AuNP solution was determined by first determining the absorbance of a blank sample. The absorbance of the citrate-AuNP solution was correct by subtracting the absorbance of the blank sample. | ||
* In order to determine the molar absorptivity, ε, this [http://pubs.acs.org/doi/suppl/10.1021/ac0702084/suppl_file/ac0702084si20070321_014144.pdf reference] was used. The peak appeared at 520 nm on the UV-VIS spectrum with a corresponding absorbance of 0.02. Using the ratio, A<sub>spr</sub>/A<sub>450</sub>, it was determined that the nanoparticle diameter was 12 nm. This diameter is associated with a molar absorptivity of 1.09x10<sup>8</sup> M<sup>-1</sup>cm<sup>-1</sup>. | * In order to determine the molar absorptivity, ε, this [http://pubs.acs.org/doi/suppl/10.1021/ac0702084/suppl_file/ac0702084si20070321_014144.pdf reference] was used. The peak appeared at 520 nm on the UV-VIS spectrum with a corresponding absorbance of 0.02. Using the ratio, A<sub>spr</sub>/A<sub>450</sub>, it was determined that the nanoparticle diameter was 12 nm. This diameter is associated with a molar absorptivity of 1.09x10<sup>8</sup> M<sup>-1</sup>cm<sup>-1</sup> as stated in the reference. | ||
* Using Beer's Law, A = εbc, the concentration of the solution was calculated to be 1.83x10<sup>-10</sup>M. |
Revision as of 10:39, 2 September 2013
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ObjectiveSynthesize two different sets of gold nanoparticles. In one set, Au3+ is reduced by a protein (bovine serum albumin, BSA) and the synthesized nanoparticle is also surrounded and stabilized by BSA. In the second set, Au3+ is reduced by citrate, and the AuNP is stabilized by citrate in solution. The BSA-AuNPs are purple in color and the citrate-AuNPs are more of a burgundy (reddish) color. From Dr. Hartings
ProcedurePart 1: BSA-AuNP Synthesis
Part 2: Citrate-AuNP Synthesis
Figures
Calculations from Part 2
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