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|style="background-color: #EEE"|[[Image: | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab: Fall 2013</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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# Add 7.19 ml deionized water to the volumetric flask. | # Add 7.19 ml deionized water to the volumetric flask. | ||
# Transfer the solution to a test tube and cap with aluminum foil. | # Transfer the solution to a test tube and cap with aluminum foil. | ||
# Heat in the oven at | # Heat in the oven at 80°C for three hours. | ||
# Transfer the solution to a plastic falcon tube. | # Transfer the solution to a plastic falcon tube. | ||
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# Determine the final concentration of gold and citrate. | # Determine the final concentration of gold and citrate. | ||
==Figures== | |||
* Spectrum from UV-VIS of 50x Diluted Citrate-AuNP Solution | |||
[[Image:UV-VIS Spectrum of UV-Vis of 50x Diluted Citrate-AuNP.png|650px|]] | |||
[[User:Matt Hartings|Matt Hartings]] In the future, your spectra are going to be much easier to read if they only have lines (and no symbols). | |||
==Calculations from Part 2== | |||
* The absorbance of the citrate-AuNP solution was determined by first determining the absorbance of a blank sample. The absorbance of the citrate-AuNP solution was correct by subtracting the absorbance of the blank sample. | |||
* In order to determine the molar absorptivity, ε, this [http://pubs.acs.org/doi/suppl/10.1021/ac0702084/suppl_file/ac0702084si20070321_014144.pdf reference] was used. The peak appeared at 520 nm on the UV-VIS spectrum with a corresponding absorbance of 0.02. Using the ratio, A<sub>spr</sub>/A<sub>450</sub>, it was determined that the nanoparticle diameter was 12 nm. This diameter is associated with a molar absorptivity of 1.09x10<sup>8</sup> M<sup>-1</sup>cm<sup>-1</sup> as stated in the reference. | |||
* Using Beer's Law, A = εbc, the concentration of the diluted solution was calculated to be 1.83x10<sup>-10</sup> M. | |||
* The concentration of gold in the original solution was calculated to be 1.97x10<sup>-9</sup> M. | |||
== | ==Observations== | ||
[[ | * The final volume of Citrate-AuNP solution was 31.0 ml. | ||
[[User:Matt Hartings|Matt Hartings]] based on this final volume (and initial concentration) what is your concentration of gold? | |||
* The progression of color change in Part 2 was light purple → dark purple → magenta → burgundy → deep red. | |||
* The final color of the BSA-AuNP solution was dark purple. |
Revision as of 09:14, 9 October 2013
Biomaterials Design Lab: Fall 2013 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveSynthesize two different sets of gold nanoparticles. In one set, Au3+ is reduced by a protein (bovine serum albumin, BSA) and the synthesized nanoparticle is also surrounded and stabilized by BSA. In the second set, Au3+ is reduced by citrate, and the AuNP is stabilized by citrate in solution. The BSA-AuNPs are purple in color and the citrate-AuNPs are more of a burgundy (reddish) color. From Dr. Hartings
ProcedurePart 1: BSA-AuNP Synthesis
Part 2: Citrate-AuNP Synthesis
Figures
Matt Hartings In the future, your spectra are going to be much easier to read if they only have lines (and no symbols). Calculations from Part 2
Observations
Matt Hartings based on this final volume (and initial concentration) what is your concentration of gold?
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