User:Carly M. Montanero/Notebook/CHEM-571/2013/08/28
From OpenWetWare
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Entry titleBiomaterials Design Lab
|
Part 1: BSA-AuNP Synthesis
- Add 1.00 ml of 2.54 mM gold solution (HAuCL4) to a 10 ml volumetric flask.
- Add 1.81 BSA (90x more gold than BSA) to the volumetric flask.
- Add 7.19 ml deionized water to the volumetric flask.
- Transfer the solution to a test tube and cap with aluminum foil.
- Heat in the oven at 80C for three hours.
- Transfer the solution to a plastic falcon tube.
Part 2: Citrate-AuNP Synthesis
- Take 50 ml of 0.254 mM gold solution (HAuCL4) from the stock solution.
- Heat the solution to boiling while stirring and covered with a watch glass.
- Add 1.5 ml of 1.01% (w/v) sodium citrate.
- Boil the solution for another 40 minutes.
- Cool to room temperature and measure the final volume.
- Determine the final concentration of gold and citrate.