User:Carly M. Montanero/Notebook/CHEM-571/2013/08/28

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Objective

Synthesize two different sets of gold nanoparticles. In one set, Au3+ is reduced by a protein (bovine serum albumin, BSA) and the synthesized nanoparticle is also surrounded and stabilized by BSA. In the second set, Au3+ is reduced by citrate, and the AuNP is stabilized by citrate in solution. The BSA-AuNPs are purple in color and the citrate-AuNPs are more of a burgundy (reddish) color.

From Dr. Hartings


Procedure

Part 1: BSA-AuNP Synthesis

  1. Add 1.00 ml of 2.54 mM gold solution (HAuCL4) to a 10 ml volumetric flask.
  2. Add 1.81 BSA (90x more gold than BSA) to the volumetric flask.
  3. Add 7.19 ml deionized water to the volumetric flask.
  4. Transfer the solution to a test tube and cap with aluminum foil.
  5. Heat in the oven at 80C for three hours.
  6. Transfer the solution to a plastic falcon tube.

Part 2: Citrate-AuNP Synthesis

  1. Take 50 ml of 0.254 mM gold solution (HAuCL4) from the stock solution.
  2. Heat the solution to boiling while stirring and covered with a watch glass.
  3. Add 1.5 ml of 1.01% (w/v) sodium citrate.
  4. Boil the solution for another 40 minutes.
  5. Cool to room temperature and measure the final volume.
  6. Determine the final concentration of gold and citrate.


Figures
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