User:Carly M. Montanero/Notebook/CHEM-571/2013/09/04: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 22: Line 22:


[[Image:inosine-UVVis-259nm.jpg|650px|]]
[[Image:inosine-UVVis-259nm.jpg|650px|]]
==Notes==
The data out group collected yesterday for inosine was systematically lower than the rest of the class data. For this reason, our inosine data was not included in the class data. The adenosine trial from yesterday was also systematically lower than the class data. We remade the stock solution and ran the sample dilutions again. The resulting data was closer to the class data, so the first trial was discarded.


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 07:15, 10 September 2013

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objective

To determine the molar absorptivity of inosine and become familiar with the fluorescence spectrometer and with protein fluorescence.

From Dr. Hartings

Procedure

A new adenosine stock was repeated, using this procedure from yesterday. An adenosine stock solution of 3.03x10-3 M was created using 0.0809 g of adenosine. From there, new dilutions were made to the same concentrations as yesterday.

Figures

Notes

The data out group collected yesterday for inosine was systematically lower than the rest of the class data. For this reason, our inosine data was not included in the class data. The adenosine trial from yesterday was also systematically lower than the class data. We remade the stock solution and ran the sample dilutions again. The resulting data was closer to the class data, so the first trial was discarded.