User:Carly M. Montanero/Notebook/CHEM-571/2013/09/11: Difference between revisions
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==Calculating the Number of Gold Atoms in Citrate-AuNP== | ==Calculating the Number of Gold Atoms in Citrate-AuNP== | ||
# The equation of the best fit line from the class data was y = 3x10<sup>-5</sup>x<sup>2</sup> + 0.0209x. | # The equation of the best fit line from the class data was y = 3x10<sup>-5</sup>x<sup>2</sup> + 0.0209x. | ||
# The average of our citrate-AuNP concentration was 0. | # The average of our corrected citrate-AuNP concentration was 0.1444 μg/mL. | ||
# Setting 0. | # Setting 0.1444 equal to y yields the equation 0.1444 = 3x10<sup>-5</sup>x<sup>2</sup> + 0.0209x. | ||
# Solving the quadratic equation gives two answers: x = - | # Solving the quadratic equation gives two answers: x = -703.51 and x = 6.842. | ||
# Disregarding the negative value, the 6. | # Disregarding the negative value, the 6.842 μg/mL corresponds to the concentration of gold. | ||
# We diluted the sample of gold by 10, so the actual concentration is 0. | # We diluted the sample of gold by 10, so the actual concentration is 0.06842 g/L. | ||
# The molecular weight of gold is 196.97 g/mol. | # The molecular weight of gold is 196.97 g/mol. | ||
# Converting the concentration of gold to molarity yields a concentration of 3. | # Converting the concentration of gold to molarity yields a concentration of 3.474x10<sup>-4</sup> M gold. | ||
# Dividing the concentration of gold (3. | # Dividing the concentration of gold (3.474x10<sup>-4</sup>) M by the concentration of citrate-AuNP ([[User:Carly_M._Montanero/Notebook/CHEM-571/2013/08/28 | 9.17×10<sup>-9</sup>M]]) gives the final number of gold atoms per citrate-AuNP molecule. | ||
# Though these calculations, there are | # Though these calculations, there are 37,884 gold atoms per citrate-AuNP molecule. | ||
==Notes== | ==Notes== |
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Objective
Procedure
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Calculating the Number of Gold Atoms in Citrate-AuNP
NotesThe UV-Vis spectra of trial 4 Bradford Assay of horseradish peroxidase had some serious errors. We discarded the data. |