User:Carly M. Montanero/Notebook/CHEM-571/2013/09/18: Difference between revisions
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[[Image:CHEM571_09.18.13_potassiumHRP_Echem_REDOX_table.png]] | [[Image:CHEM571_09.18.13_potassiumHRP_Echem_REDOX_table.png]] | ||
[[Image:CHEM571_09.18.13_potassiumHRP_Echem_REDOX_graph.png]] | [[Image:CHEM571_09.18.13_potassiumHRP_Echem_REDOX_graph.png]] | ||
==Data== | |||
The percent oxidized was determined by using the absorbance peaks at 405.32 nm: | |||
[(Absorbance Value for data point- Absorbance Min)/(Absorbance Max- Absorbance Min)]*100 | |||
* where the maximum absorbance was assumed to represent 100% oxidized and the minimum absorbance was assumed to represent 100% reduced. The percent oxidized was subtracted from 100 to calculate the percent reduced. | |||
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Revision as of 09:18, 9 October 2013
 Biomaterials Design Lab: Fall 2013
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ObjectiveTo perform a redox titration on HRP in order to determine the standard potential of this protein. ProcedureThe procedure was taken from Dr. Hartings notebook. Figures
DataThe percent oxidized was determined by using the absorbance peaks at 405.32 nm: [(Absorbance Value for data point- Absorbance Min)/(Absorbance Max- Absorbance Min)]*100
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