User:Carly M. Montanero/Notebook/CHEM-571/2013/10/09: Difference between revisions

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''' Kinetics of Luminol Oxidized by Absorption Spectrum Changes in Stopped Flow Mixer'''
''' Kinetics of Luminol Oxidized by Absorption Spectrum Changes in Stopped Flow Mixer'''
# Add 64.9 μL HRP stock, 3.31 mL luminol stock, and 6.63 mL buffer to stopped flow mixer.  
# Add 64.9 μL HRP stock, 3.31 mL luminol stock, and 6.63 mL buffer to stopped flow mixer.  
# Add 5 mL hydrogen peroxide stock and 5 mL buffer.  
# Add 5.00 mL hydrogen peroxide stock and 5.00 mL buffer to stopped flow mixer.


==Figures==
==Figures==

Revision as of 09:48, 2 October 2013

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Objective

To monitor the kinetics and yield of the horseradish peroxidase-catalyzed oxidation of luminol. These experiments will be compared to future experiments with HRP-functionalized nanoparticles. These experiments are also meant to introduce researchers to stopped-flow techniques and rapid data collection.

From Dr. Hartings

Procedure

Kinetics of Luminol Oxidized by Absorption Spectrum Changes in Stopped Flow Mixer

  1. Add 64.9 μL HRP stock, 3.31 mL luminol stock, and 6.63 mL buffer to stopped flow mixer.
  2. Add 5.00 mL hydrogen peroxide stock and 5.00 mL buffer to stopped flow mixer.

Figures

Notes

  • The stock solution of HRP was 0.77 μM.
  • The stock solution of luminol was 1.51 mM.
  • The buffer solution was 5.1 mM Tris at pH = 8.
  • The stock solution of hydrogen peroxide was 12.8 mM.



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