User:Carly M. Montanero/Notebook/CHEM-571/2013/10/09: Difference between revisions
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''' 3. Kinetics of Luminol Oxidized by Absorption Spectrum Changes in Stopped Flow Mixer''' | ''' 3. Kinetics of Luminol Oxidized by Absorption Spectrum Changes in Stopped Flow Mixer''' | ||
# Add | # Add 245.13 μL HRP stock to a volumetric flask and dilute to 10 mL with buffer. | ||
# Add mL hydrogen peroxide stock and | # Add 1.25 mL luminol stock to a volumetric flask and dilute to 10 mL with buffer. | ||
# Add 2.00 mL of diluted HRP to stock and 10.00 mL of diluted luminol to stopped flow mixer. | |||
# Add 5.00 mL hydrogen peroxide stock and 5.00 mL buffer to stopped flow mixer. | |||
# Equilibrate mixer tubes with sample. | # Equilibrate mixer tubes with sample. | ||
# Initiate | # Initiate mixing. | ||
# Using luminol and 3-aminophthalic acid spectra as endpoints, determine the kinetics of 3-aminophthalic acid synthesis. | # Using luminol and 3-aminophthalic acid spectra as endpoints, determine the kinetics of 3-aminophthalic acid synthesis. | ||
Revision as of 11:30, 2 October 2013
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ObjectiveTo monitor the kinetics and yield of the horseradish peroxidase-catalyzed oxidation of luminol. These experiments will be compared to future experiments with HRP-functionalized nanoparticles. These experiments are also meant to introduce researchers to stopped-flow techniques and rapid data collection. From Dr. Hartings Procedure2. Chemiluminescence of Luminol Oxidation Reaction Initiated by Stopped Flow Mixer
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