User:Carly M. Montanero/Notebook/CHEM-571/2013/10/16: Difference between revisions

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(0.3)(100 μL)=A2(3 mL)
(0.3)(100 μL)=A2(3 mL)
A2 = ?
A2 = ?
==Notes==
* The buffer was 5.1 mM Tris at pH 8.
* The stock concentration of luminol was 1.46 mM.
* The stock concentration of hydrogen peroxide was 44.29 mM.


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Revision as of 09:57, 16 October 2013

Biomaterials Design Lab: Fall 2013 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Procedure

  • Luminol --> final concentration such that A(330)< 1.
  • Hydrogen peroxide --> final concentration to be 50x luminol concentration
  • HRP --> 60:1 Au:HRP solutions, 100 μL
  • Buffer --> add up to 3 mL

In cuvette, luminol, buffer, and hydrogen peroxide

  • Start kinetics by adding HRP
  • What percent of HRP is still active?

Control:

  • experiment with BSA-AuNPs and citrate-AuNPs
  • what concentration of AuNPs about the same (use absorption spectra)

Data

A1V1 = A2V2 (0.3)(100 μL)=A2(3 mL) A2 = ?

Notes

  • The buffer was 5.1 mM Tris at pH 8.
  • The stock concentration of luminol was 1.46 mM.
  • The stock concentration of hydrogen peroxide was 44.29 mM.


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