User:Carly M. Montanero/Notebook/CHEM-571/2013/10/16: Difference between revisions
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==Procedure== | ==Procedure== | ||
Luminol --> final concentration such that A(330)< 1. | *Luminol --> final concentration such that A(330)< 1. | ||
Hydrogen peroxide --> final concentration to be 50x luminol concentration | *Hydrogen peroxide --> final concentration to be 50x luminol concentration | ||
HRP --> 60:1 Au:HRP solutions, 100 μL | *HRP --> 60:1 Au:HRP solutions, 100 μL | ||
Buffer --> add up to 3 mL | *Buffer --> add up to 3 mL | ||
In cuvette, luminol, buffer, and hydrogen peroxide | In cuvette, luminol, buffer, and hydrogen peroxide | ||
* Start kinetics by adding HRP | * Start kinetics by adding HRP | ||
What percent of HRP is still active? | *What percent of HRP is still active? | ||
Control: | Control: | ||
Revision as of 09:21, 16 October 2013
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Procedure
In cuvette, luminol, buffer, and hydrogen peroxide
Control:
DataA1V1 = A2V2 (0.3)(100 μL)=A2(3 mL) A2 = ? | |
