User:Carly M. Montanero/Notebook/CHEM-571/2013/10/16: Difference between revisions
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==Procedure== | ==Procedure== | ||
# Add 1.293 mL of the luminol stock and 2.131 mL of the hydrogen peroxide stock to a 10 mL volumetric flask. Dilute the solution to 10 mL with buffer. | '''Creating the Luminol-H<sub>2</sub>O<sub>2</sub> Solution''' | ||
# Add 1.293 mL of the luminol stock and 2.131 mL of the hydrogen peroxide stock to a 10 mL volumetric flask. | |||
# Dilute the solution to 10 mL with buffer. | |||
# Start kinetics measurement: | |||
:* 1 ms integration | |||
:* 10 scan average | |||
:* Set "Save the first available scan every" to 15 seconds | |||
:* Set "Stop after this amount of time" to 10 minutes | |||
:* Set "File Type" to Tab Delimited | |||
:* Just before 1 minute, add 1.5 μL of 1 nM EHNA. | |||
# Take 3 mL of the luminol-H<sub>2</sub>O<sub>2</sub> solution and place it into a cuvette. | # Take 3 mL of the luminol-H<sub>2</sub>O<sub>2</sub> solution and place it into a cuvette. | ||
# | # |
Revision as of 10:04, 16 October 2013
Biomaterials Design Lab: Fall 2013 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
PurposeTo test the activity of our HRP-NPs for the catalytic conversion of luminol. ProcedureCreating the Luminol-H2O2 Solution
In cuvette, luminol, buffer, and hydrogen peroxide
Control:
DataA1V1 = A2V2 (0.3)(100 μL)=A2(3 mL) A2 = ? Notes
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