User:Carly M. Montanero/Notebook/CHEM-571/2013/10/16

From OpenWetWare
Jump to navigationJump to search
Biomaterials Design Lab: Fall 2013 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Purpose

To test the activity of our HRP-NPs for the catalytic conversion of luminol.

Procedure

  1. Add 1.293 mL of the luminol stock and 2.131 mL of the hydrogen peroxide stock to a 10 mL volumetric flask. Dilute the solution to 10 mL with buffer.
  2. Take 3 mL of the luminol-H2O2 solution and place it into a cuvette.


  • Luminol --> final concentration such that A(330)< 1.
  • Hydrogen peroxide --> final concentration to be 50x luminol concentration
  • HRP --> 60:1 Au:HRP solutions, 100 μL
  • Buffer --> add up to 3 mL

In cuvette, luminol, buffer, and hydrogen peroxide

  • Start kinetics by adding HRP
  • What percent of HRP is still active?

Control:

  • experiment with BSA-AuNPs and citrate-AuNPs
  • what concentration of AuNPs about the same (use absorption spectra)

Data

A1V1 = A2V2 (0.3)(100 μL)=A2(3 mL) A2 = ?

Notes

  • The buffer was 5.1 mM Tris at pH 8.
  • The stock concentration of luminol was 1.46 mM.
  • The stock concentration of hydrogen peroxide was 44.29 mM.


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Carly_M._Montanero/Notebook/CHEM-571/2013/10/16&oldid=738911"