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|style="background-color: #EEE"|[[Image: | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab: Fall 2013</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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== | ==Objective== | ||
* | #To take the spectra of the pepsin-AuNP samples made yesterday | ||
#To make new HRP and Myoglobin samples | |||
==Procedure== | |||
# Protein-AuNP analysis | |||
## Take a picture and note physical description of each ratio | |||
## Place 1mL of each ratio in an 1.5mL eppendorf tube. | |||
## Centrifuge for 30 minutes (Be considerate of other uses here. Act quickly, and get as many tubes in the centrifuge as you can at once.) | |||
## Take a UV-Vis spectrum of each sample (Be considerate of other users here) | |||
## After taking the spectrum, save the sample in a fresh eppy tube. | |||
# Synthesis of HRP-AuNPs and Hemoglobin-AuNPs | |||
## Into test tube that will contain a total of 5mL add | |||
## Au solution such that the final concentration is 0.25mM | |||
## For Myoglobin make the following solutions to reflect an Au:Protein ratios | |||
### 30 | |||
### 50 | |||
### 70 | |||
### 90 | |||
### 110 | |||
### 130 | |||
### 150 | |||
### 170 | |||
### 190 | |||
### 210 | |||
## For HRP make the following solutions to reflect an Au:Protein ratios | |||
### 230 | |||
### 250 | |||
### 270 | |||
### 290 | |||
### 310 | |||
### 330 | |||
### 350 | |||
### 370 | |||
### 390 | |||
### 410 | |||
## Water up to 5mL | |||
==Figures== | |||
*How solutions were made: | |||
[[Image:CHEM571_10.23.13_solutionstable.png]] | |||
*HRP UV-Vis | |||
[[Image:11.1.2013_cmj_UVVis_HRPhigh.png]] | |||
*Myoglobin UV-Vis | |||
[[Image:11.1.2013_cmj_UVVis_myo.png]] | |||
==Notes== | |||
<u>Stock Solutions</u> | |||
# Gold | |||
## 49.3 mg HAuCl<sub>4</sub>·3H<sub>2</sub>O in 50mL water ---> 2.50mM | |||
# HRP | |||
## 6.6 mg HRP in 100 mL water ---> 1.5μM | |||
# Myoglobin | |||
## 28.7mg myoglobin in 100mL water ---> 16.9μM | |||
Revision as of 11:42, 12 November 2013
Biomaterials Design Lab: Fall 2013 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Objective
Procedure
Figures
NotesStock Solutions
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