User:Carly M. Montanero/Notebook/CHEM-571/2013/10/29: Difference between revisions
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'''Synthesizing Lysozyme AuNP and Myoglobin AuNP''' | '''Synthesizing Lysozyme AuNP and Myoglobin AuNP''' | ||
Solutions with the following Au:protein ratio | Solutions with the following Au:protein ratio were synthesized: | ||
''Gold to Lysozyme Ratio'' | ''Gold to Lysozyme Ratio'' | ||
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:*45 | :*45 | ||
:*50 | :*50 | ||
# | # As seen in Figure 1, the corresponding amount of protein was added to a 50 mL volumetric flask and filled with distilled water. The samples were made in small test tubes and capped with aluminum foil. | ||
# The samples were heated at 80°C for 4 hours. | # The samples were heated at 80°C for 4 hours. | ||
==Notes== | |||
* The final gold concentration in the solutions was 0.25 mM. | |||
Revision as of 09:54, 29 October 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveAnalysis and synthesis of more protein-AuNP materials. Each group will have a different task. One (set) of groups will be doing AA on their samples from last weed (HRP-AuNPs and pepsin-AuNPs) and this week (Myoglobin-AuNPs and HRP-AuNPs). Another (set) will take UV-Vis absorbance of their samples (this week and last week). The final (set) will synthesize new protein-AuNPs. ProcedureCreating the Gold Stock Solution Creating the Lysozyme Solution Creating the Myoglobin Solution Synthesizing Lysozyme AuNP and Myoglobin AuNP Solutions with the following Au:protein ratio were synthesized: Gold to Lysozyme Ratio
Gold to Myoglobin Ratio
Notes
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