User:Carly M. Montanero/Notebook/CHEM-571/2013/10/29: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(11 intermediate revisions by 2 users not shown)
Line 1: Line 1:
{|{{table}} width="800"
{|{{table}} width="800"
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab: Fall 2013</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
|-
Line 10: Line 10:


==Procedure==
==Procedure==
'''Creating the Gold Stock Solution'''
# Add 0.0506 g of HAuCl<sub>4</sub>·3H<sub>2</sub>O to a 50 mL volumetric flask and fill with distilled water.
# The final concentration of gold stock was 2.57 mM.
'''Creating the Lysozyme Stock Solution'''
# Add 0.010725 g of lysozyme to a 50 mL volumetric flask and fill with distilled water.
# The final concentration of lysozyme stock was 15 μM.
'''Creating the Myoglobin Stock Solution'''
# Add 0.02640 g of myoglobin to a 50 mL volumetric flask and fill with distilled water.
# The final concentration of myoglobin stock was 15 μM.
'''Synthesizing Lysozyme AuNP and Myoglobin AuNP'''
'''Synthesizing Lysozyme AuNP and Myoglobin AuNP'''
Solutions with the following Au:protein ratio and a final gold concentration of 0.25 mM were made using the information in Figure 1:
 
[[Gold to Lysozyme Ratio]]
Solutions with the following Au:protein ratio were synthesized:
30
 
50
:''Gold to Lysozyme Ratio''
70
::*30
90
::*50
110
::*70
130
::*90
150
::*110
170
::*130
190
::*150
210
::*170
[[Gold to Myoglobin Ratio]]
::*190
10
::*210
15
:''Gold to Myoglobin Ratio''
25
::*10
30
::*15
35
::*25
40
::*30
45
::*35
50
::*40
Make your stock solutions
::*45
Make your samples in small test tubes and cap with aluminum foil
::*50
Heat your samples at 80C for 4 hours
# As seen in Figure 1, the corresponding amount of protein was added to a 50 mL volumetric flask and filled with distilled water. The samples were made in small test tubes and capped with aluminum foil.
# The samples were heated at 80°C for 4 hours.
 
==Figures==
[[Image:10.29.2013_cmj_solutions1.png]]
[[Image:10.29.2013_cmj_solutions2.png]]
 
 
*Myoglobin UV-Vis
[[Image:11.5.2013_cmj_UVVis_myo.png]]
 
 
*Lysozyme UV-Vis
[[Image:11.5.2013_cmj_UVVis_lyzo.png]]
 
==Notes==
* The final gold concentration in the solutions was 0.25 mM.
 




Revision as of 11:41, 12 November 2013

Biomaterials Design Lab: Fall 2013 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objective

Analysis and synthesis of more protein-AuNP materials. Each group will have a different task. One (set) of groups will be doing AA on their samples from last weed (HRP-AuNPs and pepsin-AuNPs) and this week (Myoglobin-AuNPs and HRP-AuNPs). Another (set) will take UV-Vis absorbance of their samples (this week and last week). The final (set) will synthesize new protein-AuNPs.

Procedure

Creating the Gold Stock Solution

  1. Add 0.0506 g of HAuCl4·3H2O to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of gold stock was 2.57 mM.

Creating the Lysozyme Stock Solution

  1. Add 0.010725 g of lysozyme to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of lysozyme stock was 15 μM.

Creating the Myoglobin Stock Solution

  1. Add 0.02640 g of myoglobin to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of myoglobin stock was 15 μM.

Synthesizing Lysozyme AuNP and Myoglobin AuNP

Solutions with the following Au:protein ratio were synthesized:

Gold to Lysozyme Ratio
  • 30
  • 50
  • 70
  • 90
  • 110
  • 130
  • 150
  • 170
  • 190
  • 210
Gold to Myoglobin Ratio
  • 10
  • 15
  • 25
  • 30
  • 35
  • 40
  • 45
  • 50
  1. As seen in Figure 1, the corresponding amount of protein was added to a 50 mL volumetric flask and filled with distilled water. The samples were made in small test tubes and capped with aluminum foil.
  2. The samples were heated at 80°C for 4 hours.

Figures


  • Myoglobin UV-Vis


  • Lysozyme UV-Vis

Notes

  • The final gold concentration in the solutions was 0.25 mM.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Carly_M._Montanero/Notebook/CHEM-571/2013/10/29&oldid=756115"