User:Carly M. Montanero/Notebook/CHEM-571/2013/10/29

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(Procedure)
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==Procedure==
==Procedure==
 +
'''Creating a Gold Stock Solution'''
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#
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'''Synthesizing Lysozyme AuNP and Myoglobin AuNP'''
'''Synthesizing Lysozyme AuNP and Myoglobin AuNP'''
Solutions with the following Au:protein ratio and a final gold concentration of 0.25 mM were made using the information in Figure 1:
Solutions with the following Au:protein ratio and a final gold concentration of 0.25 mM were made using the information in Figure 1:
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[[Gold to Lysozyme Ratio]]
+
''Gold to Lysozyme Ratio''
-
30
+
:*30
-
50
+
:*50
-
70
+
:*70
-
90
+
:*90
-
110
+
:*110
-
130
+
:*130
-
150
+
:*150
-
170
+
:*170
-
190
+
:*190
-
210
+
:*210
[[Gold to Myoglobin Ratio]]
[[Gold to Myoglobin Ratio]]
-
10
+
:*10
-
15
+
:*15
-
25
+
:*25
-
30
+
:*30
-
35
+
:*35
-
40
+
:*40
-
45
+
:*45
-
50
+
:*50
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Make your stock solutions
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# Make your samples in small test tubes and cap with aluminum foil.
-
Make your samples in small test tubes and cap with aluminum foil
+
# The samples were heated at 80°C for 4 hours.
-
Heat your samples at 80C for 4 hours
+

Revision as of 11:36, 29 October 2013

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Objective

Analysis and synthesis of more protein-AuNP materials. Each group will have a different task. One (set) of groups will be doing AA on their samples from last weed (HRP-AuNPs and pepsin-AuNPs) and this week (Myoglobin-AuNPs and HRP-AuNPs). Another (set) will take UV-Vis absorbance of their samples (this week and last week). The final (set) will synthesize new protein-AuNPs.

Procedure

Creating a Gold Stock Solution

Synthesizing Lysozyme AuNP and Myoglobin AuNP Solutions with the following Au:protein ratio and a final gold concentration of 0.25 mM were made using the information in Figure 1: Gold to Lysozyme Ratio

  • 30
  • 50
  • 70
  • 90
  • 110
  • 130
  • 150
  • 170
  • 190
  • 210

Gold to Myoglobin Ratio

  • 10
  • 15
  • 25
  • 30
  • 35
  • 40
  • 45
  • 50
  1. Make your samples in small test tubes and cap with aluminum foil.
  2. The samples were heated at 80°C for 4 hours.



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