User:Carly M. Montanero/Notebook/CHEM-571/2013/10/29

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(Procedure)
(Procedure)
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==Procedure==
==Procedure==
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'''Creating a Gold Stock Solution'''
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'''Creating the Gold Stock Solution'''
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'''Creating the Lysozyme Solution'''
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'''Creating the Myoglobin Solution'''
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'''Synthesizing Lysozyme AuNP and Myoglobin AuNP'''
'''Synthesizing Lysozyme AuNP and Myoglobin AuNP'''

Revision as of 12:41, 29 October 2013

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Objective

Analysis and synthesis of more protein-AuNP materials. Each group will have a different task. One (set) of groups will be doing AA on their samples from last weed (HRP-AuNPs and pepsin-AuNPs) and this week (Myoglobin-AuNPs and HRP-AuNPs). Another (set) will take UV-Vis absorbance of their samples (this week and last week). The final (set) will synthesize new protein-AuNPs.

Procedure

Creating the Gold Stock Solution

Creating the Lysozyme Solution

Creating the Myoglobin Solution

Synthesizing Lysozyme AuNP and Myoglobin AuNP Solutions with the following Au:protein ratio and a final gold concentration of 0.25 mM were made using the information in Figure 1:

Gold to Lysozyme Ratio

  • 30
  • 50
  • 70
  • 90
  • 110
  • 130
  • 150
  • 170
  • 190
  • 210

Gold to Myoglobin Ratio

  • 10
  • 15
  • 25
  • 30
  • 35
  • 40
  • 45
  • 50
  1. Make your samples in small test tubes and cap with aluminum foil.
  2. The samples were heated at 80°C for 4 hours.



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