User:Carly M. Montanero/Notebook/CHEM-571/2013/10/29

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(Procedure)
(Procedure)
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'''Synthesizing Lysozyme AuNP and Myoglobin AuNP'''
'''Synthesizing Lysozyme AuNP and Myoglobin AuNP'''
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Solutions with the following Au:protein ratio and a final gold concentration of 0.25 mM were made using the information in Figure 1:
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Solutions with the following Au:protein ratio were synthesized:
''Gold to Lysozyme Ratio''
''Gold to Lysozyme Ratio''
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:*45
:*45
:*50
:*50
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# Make your samples in small test tubes and cap with aluminum foil.
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# As seen in Figure 1, the corresponding amount of protein was added to a 50 mL volumetric flask and filled with distilled water. The samples were made in small test tubes and capped with aluminum foil.
# The samples were heated at 80°C for 4 hours.
# The samples were heated at 80°C for 4 hours.
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 +
==Notes==
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* The final gold concentration in the solutions was 0.25 mM.
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Revision as of 11:54, 29 October 2013

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Objective

Analysis and synthesis of more protein-AuNP materials. Each group will have a different task. One (set) of groups will be doing AA on their samples from last weed (HRP-AuNPs and pepsin-AuNPs) and this week (Myoglobin-AuNPs and HRP-AuNPs). Another (set) will take UV-Vis absorbance of their samples (this week and last week). The final (set) will synthesize new protein-AuNPs.

Procedure

Creating the Gold Stock Solution

Creating the Lysozyme Solution

Creating the Myoglobin Solution

Synthesizing Lysozyme AuNP and Myoglobin AuNP Solutions with the following Au:protein ratio were synthesized:

Gold to Lysozyme Ratio

  • 30
  • 50
  • 70
  • 90
  • 110
  • 130
  • 150
  • 170
  • 190
  • 210

Gold to Myoglobin Ratio

  • 10
  • 15
  • 25
  • 30
  • 35
  • 40
  • 45
  • 50
  1. As seen in Figure 1, the corresponding amount of protein was added to a 50 mL volumetric flask and filled with distilled water. The samples were made in small test tubes and capped with aluminum foil.
  2. The samples were heated at 80°C for 4 hours.

Notes

  • The final gold concentration in the solutions was 0.25 mM.



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