User:Carly M. Montanero/Notebook/CHEM-571/2013/10/29

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(Procedure)
(Procedure)
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==Procedure==
==Procedure==
'''Creating the Gold Stock Solution'''
'''Creating the Gold Stock Solution'''
-
# Add 0.0506 g of HAuCl<sub>4</sub>·3H<sub>2</sub>O were added to a 50 mL volumetric flask and filled with distilled water.
+
# Add 0.0506 g of HAuCl<sub>4</sub>·3H<sub>2</sub>O to a 50 mL volumetric flask and fill with distilled water.
# The final concentration of gold stock was 2.57 mM.
# The final concentration of gold stock was 2.57 mM.
'''Creating the Lysozyme Solution'''
'''Creating the Lysozyme Solution'''
-
# Add  
+
# Add 0.010725 g of lysozyme to a 50 mL volumetric flask and fill with distilled water.
 +
# The final concentration of lysozyme stock was 15 μM.
'''Creating the Myoglobin Solution'''
'''Creating the Myoglobin Solution'''
-
# Add  
+
# Add 0.02640 g of myoglobin to a 50 mL volumetric flask and fill with distilled water.
 +
# The final concentration of myoglobin stock was 15 μM.
'''Synthesizing Lysozyme AuNP and Myoglobin AuNP'''
'''Synthesizing Lysozyme AuNP and Myoglobin AuNP'''

Revision as of 13:31, 29 October 2013

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Objective

Analysis and synthesis of more protein-AuNP materials. Each group will have a different task. One (set) of groups will be doing AA on their samples from last weed (HRP-AuNPs and pepsin-AuNPs) and this week (Myoglobin-AuNPs and HRP-AuNPs). Another (set) will take UV-Vis absorbance of their samples (this week and last week). The final (set) will synthesize new protein-AuNPs.

Procedure

Creating the Gold Stock Solution

  1. Add 0.0506 g of HAuCl4·3H2O to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of gold stock was 2.57 mM.

Creating the Lysozyme Solution

  1. Add 0.010725 g of lysozyme to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of lysozyme stock was 15 μM.

Creating the Myoglobin Solution

  1. Add 0.02640 g of myoglobin to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of myoglobin stock was 15 μM.

Synthesizing Lysozyme AuNP and Myoglobin AuNP Solutions with the following Au:protein ratio were synthesized:

Gold to Lysozyme Ratio
  • 30
  • 50
  • 70
  • 90
  • 110
  • 130
  • 150
  • 170
  • 190
  • 210
Gold to Myoglobin Ratio
  • 10
  • 15
  • 25
  • 30
  • 35
  • 40
  • 45
  • 50
  1. As seen in Figure 1, the corresponding amount of protein was added to a 50 mL volumetric flask and filled with distilled water. The samples were made in small test tubes and capped with aluminum foil.
  2. The samples were heated at 80°C for 4 hours.

Notes

  • The final gold concentration in the solutions was 0.25 mM.



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