User:Carly M. Montanero/Notebook/CHEM-571/2013/10/30: Difference between revisions
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== | ==Objective== | ||
* | # Synthesize Pepsin-AuNPs for reactivity tests tomorrow | ||
# Determine the Au:Protein ratio required for fiber formation for pepsin and HRP | |||
==Procedure== | |||
# Synthesis of HRP-AuNPs and Pepsin-AuNPs | |||
## Into test tube that will contain a total of 5mL add | |||
## Au solution such that the final concentration is 0.25mM | |||
## Protein solution such that the final concentration reflects the following Au:Protein ratios | |||
### 30 | |||
### 50 | |||
### 70 | |||
### 90 | |||
### 110 | |||
### 130 | |||
### 150 | |||
### 170 | |||
### 190 | |||
### 210 | |||
## Water up to 5mL | |||
Source: [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/10/22 | Dr. Hartings]] | |||
==Figures== | |||
* Solutions Made: | |||
[[Image:Solutions_10.22.2013.png]] | |||
*UV-Vis taken: gold 1:10 dilution, HRP 1:2 dilution, Pepsin Stock, BSA-HRP 1:60 supernatant | |||
[[Image:CMJ_UV-VIS_solutions_10222013.png]] | |||
Revision as of 08:20, 5 November 2013
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Objective
Procedure
Source: Dr. Hartings Figures
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