User:Carly M. Montanero/Notebook/CHEM-571/2013/10/30

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(Figures)
Line 32: Line 32:
* Solutions Made:
* Solutions Made:
[[Image:Solutions_10.22.2013.png]]
[[Image:Solutions_10.22.2013.png]]
 +
 +
 +
* Graphs of Concentration of Au vs Au:protein Ratio
 +
[[Image:10.31.2013_cmj_AA_HRP_AuNP.png]]
 +
[[Image:10.31.2013_cmj_AA_MyoAuNP.png]]
 +
[[Image:10.31.2013_cmj_AA_pepsin_AuNP.png]]
 +
 +
*UV-Vis taken: gold 1:10 dilution, HRP 1:2 dilution, Pepsin Stock, BSA-HRP 1:60 supernatant
*UV-Vis taken: gold 1:10 dilution, HRP 1:2 dilution, Pepsin Stock, BSA-HRP 1:60 supernatant
[[Image:CMJ_UV-VIS_solutions_10222013.png]]
[[Image:CMJ_UV-VIS_solutions_10222013.png]]

Revision as of 14:40, 12 November 2013

Biomaterials Design Lab: Fall 2013 Main project page
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Objective

  1. Synthesize Pepsin-AuNPs for reactivity tests tomorrow
  2. Determine the Au:Protein ratio required for fiber formation for pepsin and HRP

Procedure

  1. Synthesis of HRP-AuNPs and Pepsin-AuNPs
    1. Into test tube that will contain a total of 5mL add
    2. Au solution such that the final concentration is 0.25mM
    3. Protein solution such that the final concentration reflects the following Au:Protein ratios
      1. 30
      2. 50
      3. 70
      4. 90
      5. 110
      6. 130
      7. 150
      8. 170
      9. 190
      10. 210
    4. Water up to 5mL

Source: Dr. Hartings

Figures

  • Solutions Made:

Image:Solutions_10.22.2013.png


  • Graphs of Concentration of Au vs Au:protein Ratio

Image:10.31.2013_cmj_AA_HRP_AuNP.png Image:10.31.2013_cmj_AA_MyoAuNP.png Image:10.31.2013_cmj_AA_pepsin_AuNP.png


  • UV-Vis taken: gold 1:10 dilution, HRP 1:2 dilution, Pepsin Stock, BSA-HRP 1:60 supernatant

Image:CMJ_UV-VIS_solutions_10222013.png




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