User:Carly M. Montanero/Notebook/CHEM-571/2013/11/06: Difference between revisions
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'''Creating the Gold Stock Solution''' | '''Creating the Gold Stock Solution''' | ||
# Add 0.0524 g of HAuCl<sub>4</sub>·3H<sub>2</sub>O to a 50 mL volumetric flask and fill with distilled water. | # Add 0.0524 g of HAuCl<sub>4</sub>·3H<sub>2</sub>O to a 50 mL volumetric flask and fill with distilled water. | ||
# The final concentration of gold stock was 2. | # The final concentration of gold stock was 2.66 mM. | ||
'''Creating the Lysozyme Stock Solution''' | '''Creating the Lysozyme Stock Solution''' | ||
# Add 0. | # Add 0.0717 g of lysozyme to a 50 mL volumetric flask and fill with distilled water. | ||
# The final concentration of lysozyme stock was | # The final concentration of lysozyme stock was 100.28 μM. | ||
'''Creating the Hemoglobin Stock Solution''' | '''Creating the Hemoglobin Stock Solution''' | ||
# Add 0. | # Add 0.0117 g of hemoglobin to a 50 mL volumetric flask and fill with distilled water. | ||
# The final concentration of hemoglobin stock was 15 μM. | # The final concentration of hemoglobin stock was 15 μM. | ||
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::*50 | ::*50 | ||
# As seen below, the corresponding amount of protein was added to a 50 mL volumetric flask and filled with distilled water. The samples were made in small test tubes and capped with aluminum foil. | |||
# The samples were heated at 80°C for 4 hours. | |||
==Data== | |||
*Lysozyme Solutions | |||
[[Image:11.6.2013_cmj_solutions_lyz.png]] | |||
*Hemoglobin Soultions | |||
[[Image:11.6.2013_cmj_solutions_heme.png]] | |||
Revision as of 10:10, 4 February 2014
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ObjectiveTo synthesize hemoglobin-AuNPs and lysozyme-AuNPs. ProcedureCreating the Gold Stock Solution
Creating the Lysozyme Stock Solution
Creating the Hemoglobin Stock Solution
Data
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