User:Carly M. Montanero/Notebook/CHEM-571/2013/11/06

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(Autocreate 2013/11/06 Entry for User:Carly_M._Montanero/Notebook/CHEM-571)
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==Entry title==
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==Objective==
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* Insert content here...
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To synthesize hemoglobin-AuNPs and lysozyme-AuNPs.
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==Procedure==
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'''Creating the Gold Stock Solution'''
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# Add 0.0506 g of HAuCl<sub>4</sub>·3H<sub>2</sub>O to a 50 mL volumetric flask and fill with distilled water.
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# The final concentration of gold stock was 2.57 mM.
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'''Creating the Lysozyme Stock Solution'''
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# Add 0.010725 g of lysozyme to a 50 mL volumetric flask and fill with distilled water.
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# The final concentration of lysozyme stock was 15 μM.
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'''Creating the Myoglobin Stock Solution'''
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# Add 0.02640 g of myoglobin to a 50 mL volumetric flask and fill with distilled water.
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# The final concentration of myoglobin stock was 15 μM.
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 +
 
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Solutions with the following Au:protein ratio were synthesized:
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:''Gold to Hemoglobin Ratio''
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::*30
 +
::*50
 +
::*70
 +
::*90
 +
::*110
 +
::*130
 +
::*150
 +
::*170
 +
::*190
 +
::*210
 +
:''Gold to Lysozyme Ratio''
 +
::*5
 +
::*10
 +
::*15
 +
::*20
 +
::*25
 +
::*30
 +
::*35
 +
::*40
 +
::*45
 +
::*50
 +
 

Revision as of 13:31, 6 November 2013

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Objective

To synthesize hemoglobin-AuNPs and lysozyme-AuNPs.

Procedure

Creating the Gold Stock Solution

  1. Add 0.0506 g of HAuCl4·3H2O to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of gold stock was 2.57 mM.

Creating the Lysozyme Stock Solution

  1. Add 0.010725 g of lysozyme to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of lysozyme stock was 15 μM.

Creating the Myoglobin Stock Solution

  1. Add 0.02640 g of myoglobin to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of myoglobin stock was 15 μM.


Solutions with the following Au:protein ratio were synthesized:

Gold to Hemoglobin Ratio
  • 30
  • 50
  • 70
  • 90
  • 110
  • 130
  • 150
  • 170
  • 190
  • 210
Gold to Lysozyme Ratio
  • 5
  • 10
  • 15
  • 20
  • 25
  • 30
  • 35
  • 40
  • 45
  • 50



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