User:Carly M. Montanero/Notebook/CHEM-571/2013/11/12: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(6 intermediate revisions by the same user not shown)
Line 1: Line 1:
{|{{table}} width="800"
{|{{table}} width="800"
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab: Fall 2013</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
|-
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Objective==
==Objective==
To synthesize hemoglobin-AuNPs and lysozyme-AuNPs.
To take UV-Vis spectra of protein-AuNP samples.


==Procedure==
==Procedure==
'''Creating the Gold Stock Solution'''
# Centrifuge 1mL of the sample for 30 min at max rpm.
# Add 0.0506 g of HAuCl<sub>4</sub>·3H<sub>2</sub>O to a 50 mL volumetric flask and fill with distilled water.
# Take UV-Vis measurements of the samples.
# The final concentration of gold stock was 2.57 mM.
 
'''Creating the Lysozyme Stock Solution'''
# Add 0.010725 g of lysozyme to a 50 mL volumetric flask and fill with distilled water.
# The final concentration of lysozyme stock was 15 μM.
 
'''Creating the Myoglobin Stock Solution'''
# Add 0.02640 g of myoglobin to a 50 mL volumetric flask and fill with distilled water.
# The final concentration of myoglobin stock was 15 μM.




Solutions with the following Au:protein ratio were synthesized:
Solutions with the following Au:protein ratio were run on the UV-Vis:


:''Gold to Hemoglobin Ratio''
:''Gold to Hemoglobin Ratio''
Line 47: Line 39:
::*45
::*45
::*50
::*50
==Figures==
[[Image:11.5.13_Lyz_uvvis_cmj.png]]
[[Image:11.12.13_hemo_uvvis_cmj.png]]


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 10:44, 19 November 2013

Biomaterials Design Lab: Fall 2013 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objective

To take UV-Vis spectra of protein-AuNP samples.

Procedure

  1. Centrifuge 1mL of the sample for 30 min at max rpm.
  2. Take UV-Vis measurements of the samples.


Solutions with the following Au:protein ratio were run on the UV-Vis:

Gold to Hemoglobin Ratio
  • 30
  • 50
  • 70
  • 90
  • 110
  • 130
  • 150
  • 170
  • 190
  • 210
Gold to Lysozyme Ratio
  • 5
  • 10
  • 15
  • 20
  • 25
  • 30
  • 35
  • 40
  • 45
  • 50

Figures




Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Carly_M._Montanero/Notebook/CHEM-571/2013/11/12&oldid=757660"