User:Carly M. Montanero/Notebook/CHEM-571/2013/11/12: Difference between revisions

Revision as of 10:30, 6 November 2013

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-==Objective==
-To synthesize hemoglobin-AuNPs and lysozyme-AuNPs.
-==Procedure==
-'''Creating the Gold Stock Solution'''
-# Add 0.0506 g of HAuCl<sub>4</sub>·3H<sub>2</sub>O to a 50 mL volumetric flask and fill with distilled water.
-# The final concentration of gold stock was 2.57 mM.
-'''Creating the Lysozyme Stock Solution'''
-# Add 0.010725 g of lysozyme to a 50 mL volumetric flask and fill with distilled water.
-# The final concentration of lysozyme stock was 15 μM.
-'''Creating the Myoglobin Stock Solution'''
-# Add 0.02640 g of myoglobin to a 50 mL volumetric flask and fill with distilled water.
-# The final concentration of myoglobin stock was 15 μM.
-Solutions with the following Au:protein ratio were synthesized:
-:''Gold to Hemoglobin Ratio''
-::*30
-::*50
-::*70
-::*90
-::*110
-::*130
-::*150
-::*170
-::*190
-::*210
-:''Gold to Lysozyme Ratio''
-::*5
-::*10
-::*15
-::*20
-::*25
-::*30
-::*35
-::*40
-::*45
-::*50
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