User:Carly M. Montanero/Notebook/CHEM-571/2013/11/12: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 10: Line 10:


==Procedure==
==Procedure==
'''Creating the Gold Stock Solution'''
# Add 0.0506 g of HAuCl<sub>4</sub>·3H<sub>2</sub>O to a 50 mL volumetric flask and fill with distilled water.
# The final concentration of gold stock was 2.57 mM.


'''Creating the Lysozyme Stock Solution'''
# Add 0.010725 g of lysozyme to a 50 mL volumetric flask and fill with distilled water.
# The final concentration of lysozyme stock was 15 μM.
'''Creating the Myoglobin Stock Solution'''
# Add 0.02640 g of myoglobin to a 50 mL volumetric flask and fill with distilled water.
# The final concentration of myoglobin stock was 15 μM.
Solutions with the following Au:protein ratio were synthesized:
:''Gold to Hemoglobin Ratio''
::*30
::*50
::*70
::*90
::*110
::*130
::*150
::*170
::*190
::*210
:''Gold to Lysozyme Ratio''
::*5
::*10
::*15
::*20
::*25
::*30
::*35
::*40
::*45
::*50


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 10:17, 6 November 2013

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objective

To synthesize hemoglobin-AuNPs and lysozyme-AuNPs.

Procedure

Creating the Gold Stock Solution

  1. Add 0.0506 g of HAuCl4·3H2O to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of gold stock was 2.57 mM.

Creating the Lysozyme Stock Solution

  1. Add 0.010725 g of lysozyme to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of lysozyme stock was 15 μM.

Creating the Myoglobin Stock Solution

  1. Add 0.02640 g of myoglobin to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of myoglobin stock was 15 μM.

Solutions with the following Au:protein ratio were synthesized:

Gold to Hemoglobin Ratio
  • 30
  • 50
  • 70
  • 90
  • 110
  • 130
  • 150
  • 170
  • 190
  • 210
Gold to Lysozyme Ratio
  • 5
  • 10
  • 15
  • 20
  • 25
  • 30
  • 35
  • 40
  • 45
  • 50


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Carly_M._Montanero/Notebook/CHEM-571/2013/11/12&oldid=754035"