User:Carly M. Montanero/Notebook/CHEM-571/2013/11/12

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Procedure)
(Procedure)
Line 21: Line 21:
# Add 0.02640 g of myoglobin to a 50 mL volumetric flask and fill with distilled water.  
# Add 0.02640 g of myoglobin to a 50 mL volumetric flask and fill with distilled water.  
# The final concentration of myoglobin stock was 15 μM.
# The final concentration of myoglobin stock was 15 μM.
 +
Solutions with the following Au:protein ratio were synthesized:
Solutions with the following Au:protein ratio were synthesized:

Revision as of 12:17, 6 November 2013

Project name Main project page
Previous entry      Next entry

Objective

To synthesize hemoglobin-AuNPs and lysozyme-AuNPs.

Procedure

Creating the Gold Stock Solution

  1. Add 0.0506 g of HAuCl4·3H2O to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of gold stock was 2.57 mM.

Creating the Lysozyme Stock Solution

  1. Add 0.010725 g of lysozyme to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of lysozyme stock was 15 μM.

Creating the Myoglobin Stock Solution

  1. Add 0.02640 g of myoglobin to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of myoglobin stock was 15 μM.


Solutions with the following Au:protein ratio were synthesized:

Gold to Hemoglobin Ratio
  • 30
  • 50
  • 70
  • 90
  • 110
  • 130
  • 150
  • 170
  • 190
  • 210
Gold to Lysozyme Ratio
  • 5
  • 10
  • 15
  • 20
  • 25
  • 30
  • 35
  • 40
  • 45
  • 50


Personal tools