User:Carly M. Montanero/Notebook/CHEM-571/2013/11/12

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(Procedure)
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==Objective==
 
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To synthesize hemoglobin-AuNPs and lysozyme-AuNPs.
 
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==Procedure==
 
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'''Creating the Gold Stock Solution'''
 
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# Add 0.0506 g of HAuCl<sub>4</sub>·3H<sub>2</sub>O to a 50 mL volumetric flask and fill with distilled water.
 
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# The final concentration of gold stock was 2.57 mM.
 
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'''Creating the Lysozyme Stock Solution'''
 
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# Add 0.010725 g of lysozyme to a 50 mL volumetric flask and fill with distilled water.
 
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# The final concentration of lysozyme stock was 15 μM.
 
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'''Creating the Myoglobin Stock Solution'''
 
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# Add 0.02640 g of myoglobin to a 50 mL volumetric flask and fill with distilled water.
 
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# The final concentration of myoglobin stock was 15 μM.
 
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Solutions with the following Au:protein ratio were synthesized:
 
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:''Gold to Hemoglobin Ratio''
 
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::*30
 
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::*50
 
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::*70
 
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::*90
 
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::*110
 
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::*130
 
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::*150
 
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::*170
 
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::*190
 
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::*210
 
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:''Gold to Lysozyme Ratio''
 
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::*5
 
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::*10
 
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::*15
 
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::*20
 
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::*25
 
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::*30
 
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::*35
 
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::*40
 
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::*45
 
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::*50
 
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Revision as of 13:30, 6 November 2013

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