User:Carly M. Montanero/Notebook/CHEM-571/2013/11/13

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Revision as of 14:05, 13 November 2013

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Objective

To run atomic absorption (AA) on our lysozyme and myoglobin and hemoglobin samples.

Procedure

Creating the Gold Stock Solution

  1. Add 0.0506 g of HAuCl4·3H2O to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of gold stock was 2.57 mM.


Atomic Absorption Samples

Solutions with the following Au:protein ratio were run on the AA:

Gold to Hemoglobin Ratio
  • 30
  • 50
  • 70
  • 90
  • 110
  • 130
  • 150
  • 170
  • 190
  • 210
Gold to Lysozyme Ratio
  • 5
  • 10
  • 15
  • 20
  • 25
  • 30
  • 35
  • 40
  • 45
  • 50
Gold to Lysozyme Ratio
  • 30
  • 50
  • 70
  • 90
  • 110
  • 130
  • 150
  • 170
  • 190
  • 210
Gold to Myoglobin Ratio
  • 10
  • 15
  • 25
  • 30
  • 35
  • 40
  • 45
  • 50

Figures


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