User:Carly M. Montanero/Notebook/CHEM-571/2013/11/19: Difference between revisions

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'''Creating the Gold Stock Solution'''
'''Creating the Gold Stock Solution'''
# Add 0.0524 g of HAuCl<sub>4</sub>·3H<sub>2</sub>O to a 50 mL volumetric flask and fill with distilled water.
# Add 0.0345 g of HAuCl<sub>4</sub>·3H<sub>2</sub>O to a 50 mL volumetric flask and fill with distilled water.
# The final concentration of gold stock was 2.66 mM.
# The final concentration of gold stock was 3.59 mM.


'''Creating the Invertase Stock Solution'''
'''Creating the Invertase Stock Solution'''
# Add 0.0717 g of lysozyme to a 50 mL volumetric flask and fill with distilled water.
# Add 0.1015 g of invertase to a 50 mL volumetric flask and fill with distilled water.
# The final concentration of lysozyme stock was 100.28 μM.
# The final concentration of invertase stock was 30.07μM


'''Creating the BSA Stock Solution'''
'''Creating the BSA Stock Solution'''
# Add 0.0117 g of hemoglobin to a 50 mL volumetric flask and fill with distilled water.  
# Add 0.0432 g of BSA to a 50 mL volumetric flask and fill with distilled water.  
# The final concentration of hemoglobin stock was 15 μM.
# The final concentration of BSA stock was 21.8 mM.




Solutions with the following Au:protein ratio were synthesized:
Solutions with the following Au:protein ratio were synthesized:


:''Gold to Hemoglobin Ratio''
:''Gold to Invertase Ratio''
::*30
::*30
::*50
::*50
Line 37: Line 37:
::*190
::*190
::*210
::*210
:''Gold to Lysozyme Ratio''
:''Gold to BSA Ratio''
::*5
::*105
::*10
::*110
::*15
::*115
::*20
::*120
::*25
::*125
::*30
::*130
::*35
::*135
::*40
::*140
::*45
::*145
::*50
::*150
 
 
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__NOTOC__
__NOTOC__

Revision as of 11:13, 19 November 2013

Biomaterials Design Lab: Fall 2013 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objective

To synthesize invertase-AuNPs and BSA-AuNPs.

Procedure

Creating the Gold Stock Solution

  1. Add 0.0345 g of HAuCl4·3H2O to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of gold stock was 3.59 mM.

Creating the Invertase Stock Solution

  1. Add 0.1015 g of invertase to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of invertase stock was 30.07μM

Creating the BSA Stock Solution

  1. Add 0.0432 g of BSA to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of BSA stock was 21.8 mM.


Solutions with the following Au:protein ratio were synthesized:

Gold to Invertase Ratio
  • 30
  • 50
  • 70
  • 90
  • 110
  • 130
  • 150
  • 170
  • 190
  • 210
Gold to BSA Ratio
  • 105
  • 110
  • 115
  • 120
  • 125
  • 130
  • 135
  • 140
  • 145
  • 150



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