User:Carly M. Montanero/Notebook/CHEM-571/2013/11/19

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(Autocreate 2013/11/19 Entry for User:Carly_M._Montanero/Notebook/CHEM-571)
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==Entry title==
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==Objective==
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* Insert content here...
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To synthesize invertase-AuNPs and BSA-AuNPs.
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==Procedure==
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'''Creating the Gold Stock Solution'''
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# Add 0.0524 g of HAuCl<sub>4</sub>·3H<sub>2</sub>O to a 50 mL volumetric flask and fill with distilled water.
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# The final concentration of gold stock was 2.66 mM.
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'''Creating the Invertase Stock Solution'''
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# Add 0.0717 g of lysozyme to a 50 mL volumetric flask and fill with distilled water.
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# The final concentration of lysozyme stock was 100.28 μM.
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'''Creating the BSA Stock Solution'''
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# Add 0.0117 g of hemoglobin to a 50 mL volumetric flask and fill with distilled water.
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# The final concentration of hemoglobin stock was 15 μM.
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Solutions with the following Au:protein ratio were synthesized:
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:''Gold to Hemoglobin Ratio''
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::*30
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::*50
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::*70
 +
::*90
 +
::*110
 +
::*130
 +
::*150
 +
::*170
 +
::*190
 +
::*210
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:''Gold to Lysozyme Ratio''
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::*5
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::*10
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::*15
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::*20
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::*25
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::*30
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::*35
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::*40
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::*45
 +
::*50
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Revision as of 13:41, 19 November 2013

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Objective

To synthesize invertase-AuNPs and BSA-AuNPs.

Procedure

Creating the Gold Stock Solution

  1. Add 0.0524 g of HAuCl4·3H2O to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of gold stock was 2.66 mM.

Creating the Invertase Stock Solution

  1. Add 0.0717 g of lysozyme to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of lysozyme stock was 100.28 μM.

Creating the BSA Stock Solution

  1. Add 0.0117 g of hemoglobin to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of hemoglobin stock was 15 μM.


Solutions with the following Au:protein ratio were synthesized:

Gold to Hemoglobin Ratio
  • 30
  • 50
  • 70
  • 90
  • 110
  • 130
  • 150
  • 170
  • 190
  • 210
Gold to Lysozyme Ratio
  • 5
  • 10
  • 15
  • 20
  • 25
  • 30
  • 35
  • 40
  • 45
  • 50


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