User:Carly M. Montanero/Notebook/CHEM-571/2013/11/19

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(Procedure)
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'''Creating the Gold Stock Solution'''
'''Creating the Gold Stock Solution'''
-
# Add 0.0524 g of HAuCl<sub>4</sub>·3H<sub>2</sub>O to a 50 mL volumetric flask and fill with distilled water.
+
# Add 0.0345 g of HAuCl<sub>4</sub>·3H<sub>2</sub>O to a 50 mL volumetric flask and fill with distilled water.
-
# The final concentration of gold stock was 2.66 mM.
+
# The final concentration of gold stock was 3.59 mM.
'''Creating the Invertase Stock Solution'''
'''Creating the Invertase Stock Solution'''
-
# Add 0.0717 g of lysozyme to a 50 mL volumetric flask and fill with distilled water.
+
# Add 0.1015 g of invertase to a 50 mL volumetric flask and fill with distilled water.
-
# The final concentration of lysozyme stock was 100.28 μM.
+
# The final concentration of invertase stock was 30.07μM
'''Creating the BSA Stock Solution'''
'''Creating the BSA Stock Solution'''
-
# Add 0.0117 g of hemoglobin to a 50 mL volumetric flask and fill with distilled water.  
+
# Add 0.0432 g of BSA to a 50 mL volumetric flask and fill with distilled water.  
-
# The final concentration of hemoglobin stock was 15 μM.
+
# The final concentration of BSA stock was 21.8 mM.
Solutions with the following Au:protein ratio were synthesized:
Solutions with the following Au:protein ratio were synthesized:
-
:''Gold to Hemoglobin Ratio''
+
:''Gold to Invertase Ratio''
::*30
::*30
::*50
::*50
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::*190
::*190
::*210
::*210
-
:''Gold to Lysozyme Ratio''
+
:''Gold to BSA Ratio''
-
::*5
+
::*105
-
::*10
+
::*110
-
::*15
+
::*115
-
::*20
+
::*120
-
::*25
+
::*125
-
::*30
+
::*130
-
::*35
+
::*135
-
::*40
+
::*140
-
::*45
+
::*145
-
::*50
+
::*150
 +
 
 +
 
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__NOTOC__
__NOTOC__

Revision as of 14:13, 19 November 2013

Biomaterials Design Lab: Fall 2013 Main project page
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Objective

To synthesize invertase-AuNPs and BSA-AuNPs.

Procedure

Creating the Gold Stock Solution

  1. Add 0.0345 g of HAuCl4·3H2O to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of gold stock was 3.59 mM.

Creating the Invertase Stock Solution

  1. Add 0.1015 g of invertase to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of invertase stock was 30.07μM

Creating the BSA Stock Solution

  1. Add 0.0432 g of BSA to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of BSA stock was 21.8 mM.


Solutions with the following Au:protein ratio were synthesized:

Gold to Invertase Ratio
  • 30
  • 50
  • 70
  • 90
  • 110
  • 130
  • 150
  • 170
  • 190
  • 210
Gold to BSA Ratio
  • 105
  • 110
  • 115
  • 120
  • 125
  • 130
  • 135
  • 140
  • 145
  • 150



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