User:Carly M. Montanero/Notebook/CHEM-571/2013/11/19

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(Procedure)
Current revision (13:42, 20 November 2013) (view source)
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==Notes==
==Notes==
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* The BSA concentration was incorrectly calculated. We will remake the solutions tomorrow.  
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* The BSA stock concentration was calculated incorrectly. We will remake the BSA-AuNP solutions tomorrow.  
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Current revision

Biomaterials Design Lab: Fall 2013 Main project page
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Objective

To synthesize invertase-AuNPs and BSA-AuNPs.

Procedure

Creating the Gold Stock Solution

  1. Add 0.0345 g of HAuCl4·3H2O to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of gold stock was 3.59 mM.

Creating the Invertase Stock Solution

  1. Add 0.1015 g of invertase to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of invertase stock was 30.07μM

Creating the BSA Stock Solution

  1. Add 0.0432 g of BSA to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of BSA stock was 21.8 mM.


Solutions with the following Au:protein ratio were synthesized:

Gold to Invertase Ratio
  • 30
  • 50
  • 70
  • 90
  • 110
  • 130
  • 150
  • 170
  • 190
  • 210
Gold to BSA Ratio
  • 105
  • 110
  • 115
  • 120
  • 125
  • 130
  • 135
  • 140
  • 145
  • 150
  1. As seen below, the corresponding amount of protein was added to a 50 mL volumetric flask and filled with distilled water. The samples were made in small test tubes and capped with aluminum foil.
  2. The samples were heated at 80°C for 4 hours.

Figures

Image:11.19.2013_cmj_solutions1.png Image:11.19.2013_cmj_solutions2.png


Notes

  • The BSA stock concentration was calculated incorrectly. We will remake the BSA-AuNP solutions tomorrow.


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