User:Carly M. Montanero/Notebook/CHEM-571/2013/11/20: Difference between revisions

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{|{{table}} width="800"
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab: Fall 2013</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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'''Preparing Invertase-AuNP Samples for UV-Vis'''
'''Preparing Invertase-AuNP Samples for UV-Vis'''
# Centrifuge 1mL of the sample for 30 min at max rpm.
# Centrifuge 1mL of the sample for 30 min at max rpm.
# Take UV-Vis measurements of the samples.
# Take UV-Vis measurements of the samples.]
 
 
Solutions with the following Au:protein ratio were run on the UV-Vis:
:''Gold to Invertase Ratio''
::*30
::*50
::*70
::*90
::*110
::*130
::*150
::*170
::*190
::*210




'''Creating the Gold Stock Solution'''
'''Creating the Gold Stock Solution'''
# Add 0.0562 g of HAuCl<sub>4</sub>·3H<sub>2</sub>O to a 50 mL volumetric flask and fill with distilled water.
# Add 0.0562 g of HAuCl4·3H2O to a 50 mL volumetric flask and fill with distilled water.
# The final concentration of gold stock was 2.85 mM.
# The final concentration of gold stock was 2.85 mM.




'''Creating the BSA Stock Solution'''
'''Creating the BSA Stock Solution'''
# Add 0.0432 g of BSA to a 50 mL volumetric flask and fill with distilled water.  
# Add 0.0260 g of BSA to a 25 mL volumetric flask and fill with distilled water.  
# The final concentration of BSA stock was 21.8 mM.
# The final concentration of BSA stock was 15.65 μM.
 


Solutions with the following Au:protein ratio were synthesized:
Solutions with the following Au:protein ratio were synthesized:
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::*145
::*145
::*150
::*150
# As seen below, the corresponding amount of protein was added to a 50 mL volumetric flask and filled with distilled water. The samples were made in small test tubes and capped with aluminum foil.
# The samples were heated at 80°C for 4 hours.
[[Image:11.20.2013_cmj_solutions1.png]]
==Figures==
[[Image:11.20.2013_cmj_Invertase_UVvis.png]]


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Revision as of 11:02, 3 December 2013

Biomaterials Design Lab: Fall 2013 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objective

To synthesize BSA-AuNPs and run UV-Vis on the invertase-AuNP solutions created yesterday.

Procedure

Preparing Invertase-AuNP Samples for UV-Vis

  1. Centrifuge 1mL of the sample for 30 min at max rpm.
  2. Take UV-Vis measurements of the samples.]


Solutions with the following Au:protein ratio were run on the UV-Vis:

Gold to Invertase Ratio
  • 30
  • 50
  • 70
  • 90
  • 110
  • 130
  • 150
  • 170
  • 190
  • 210


Creating the Gold Stock Solution

  1. Add 0.0562 g of HAuCl4·3H2O to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of gold stock was 2.85 mM.


Creating the BSA Stock Solution

  1. Add 0.0260 g of BSA to a 25 mL volumetric flask and fill with distilled water.
  2. The final concentration of BSA stock was 15.65 μM.


Solutions with the following Au:protein ratio were synthesized:

Gold to BSA Ratio
  • 105
  • 110
  • 115
  • 120
  • 125
  • 130
  • 135
  • 140
  • 145
  • 150


  1. As seen below, the corresponding amount of protein was added to a 50 mL volumetric flask and filled with distilled water. The samples were made in small test tubes and capped with aluminum foil.
  2. The samples were heated at 80°C for 4 hours.


Figures



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