User:Carly M. Montanero/Notebook/CHEM-571/2013/11/20

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(Autocreate 2013/11/20 Entry for User:Carly_M._Montanero/Notebook/CHEM-571)
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==Entry title==
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==Objective==
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* Insert content here...
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To synthesize BSA-AuNPs and run UV-Vis on the invertase-AuNP solutions created yesterday.
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==Procedure==
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'''Preparing Invertase-AuNP Samples for UV-Vis'''
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# Centrifuge 1mL of the sample for 30 min at max rpm.
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# Take UV-Vis measurements of the samples.
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'''Creating the Gold Stock Solution'''
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# Add 0.0562 g of HAuCl<sub>4</sub>·3H<sub>2</sub>O to a 50 mL volumetric flask and fill with distilled water.
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# The final concentration of gold stock was 2.85 mM.
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'''Creating the BSA Stock Solution'''
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# Add 0.0432 g of BSA to a 50 mL volumetric flask and fill with distilled water.
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# The final concentration of BSA stock was 21.8 mM.
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Solutions with the following Au:protein ratio were synthesized:
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:''Gold to BSA Ratio''
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::*105
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::*110
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::*115
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::*120
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::*125
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::*130
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::*135
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::*140
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::*145
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::*150
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Revision as of 13:35, 20 November 2013

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Objective

To synthesize BSA-AuNPs and run UV-Vis on the invertase-AuNP solutions created yesterday.

Procedure

Preparing Invertase-AuNP Samples for UV-Vis

  1. Centrifuge 1mL of the sample for 30 min at max rpm.
  2. Take UV-Vis measurements of the samples.


Creating the Gold Stock Solution

  1. Add 0.0562 g of HAuCl4·3H2O to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of gold stock was 2.85 mM.


Creating the BSA Stock Solution

  1. Add 0.0432 g of BSA to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of BSA stock was 21.8 mM.

Solutions with the following Au:protein ratio were synthesized:

Gold to BSA Ratio
  • 105
  • 110
  • 115
  • 120
  • 125
  • 130
  • 135
  • 140
  • 145
  • 150


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