User:Carly M. Montanero/Notebook/CHEM-571/2014/01/29: Difference between revisions
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# Take 1 mL aliquots from each test tube and place in 1.5 mL tubes. | # Take 1 mL aliquots from each test tube and place in 1.5 mL tubes. | ||
===Chelators=== | |||
'''Creating Stock Solutions of Chelators''' | '''Creating Stock Solutions of Chelators''' | ||
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[[Image:1.29.data.chelator.add]] | [[Image:1.29.data.chelator.add]] | ||
===pH=== | |||
'''Creating Buffer Solutions''' | |||
''Citric Acid'' | |||
# Add 42.02 g of citric acid in 180 mL distilled water. | |||
# Titrate to pH 3.0 with monovalent strong acid/base if necessary. | |||
# Fill up to 200 mL with distilled water. | |||
''Phosphate'' | |||
# Add 0.0908 mol of sodium phosphate monobasic to 0.1091 mol of sodium phosphate dibasic. | |||
# Fill up to 200 mL with distilled water. | |||
''Tris'' | |||
# Dissolve 24.228 g Tris base in 180 mL distilled water. | |||
# Titrate to pH 9.0 with monovalent strong acid/base if necessary. | |||
# Fill up to 200 mL with distilled water. | |||
''MES'' | |||
# Dissole 39.04 g MES in 180 mL distilled water. | |||
# Titrate to pH 5.2 with monovalent strong acid/base if necessary. | |||
# Fill up to 200 mL with distilled water. | |||
''MOPS'' | |||
''CHES'' | |||
# Dissolve 41.46 g CHES in 180 mL distilled water. | |||
# Titrate to pH 9 with monovalent strong acid/base if necessary. | |||
# Fill up to 200 mL with distilled water. | |||
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Revision as of 13:22, 28 January 2014
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveTo test fiber dissolution by adding various chelators and changing the pH. Procedure
ChelatorsCreating Stock Solutions of Chelators Adding Chelators to Lysozyme-AuNP Solutions
pHCreating Buffer Solutions Citric Acid
Phosphate
Tris
MES
MOPS CHES
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