User:Carly M. Montanero/Notebook/CHEM-571/2014/01/29: Difference between revisions
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'''Creating 1 M Buffer Solutions''' | '''Creating 1 M Buffer Solutions''' | ||
''Citric Acid pH=2.99'' | ''Citric Acid: pH=2.99'' | ||
# Add 42.02 g of citric acid in 180 mL distilled water. | # Add 42.02 g of citric acid in 180 mL distilled water. | ||
# Titrate to pH 3.0 with monovalent strong acid/base if necessary. | # Titrate to pH 3.0 with monovalent strong acid/base if necessary. | ||
# Fill up to 200 mL with distilled water. | # Fill up to 200 mL with distilled water. | ||
''Phosphate pH=6.81'' | ''Phosphate: pH=6.81'' | ||
# Add 0.0908 mol of sodium phosphate monobasic to 0.1091 mol of sodium phosphate dibasic. | # Add 0.0908 mol of sodium phosphate monobasic to 0.1091 mol of sodium phosphate dibasic. | ||
# Fill up to 200 mL with distilled water. | # Fill up to 200 mL with distilled water. |
Revision as of 12:24, 29 January 2014
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ObjectiveTo create stock solutions for changing pH. Procedure
ChelatorsCreating Stock Solutions of Chelators Adding Chelators to Lysozyme-AuNP Solutions
pHCreating 1 M Buffer Solutions Citric Acid: pH=2.99
Phosphate: pH=6.81
Tris
MES
HEPES
CHES
Adding Buffers to Lysozyme-AuNP Solutions
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